Strategy of cDNA sequence

Please submit the following:
 A completed assignment cover page (provided)
 A 150 word description of the function of your allocated protein
 A 150 word explanation of your cloning strategy
 The coding region of your allocated cDNA sequence (not the full ncbi entry!)
 A restriction map of your coding region (include a list of non-cutting enzymes)
 Plasmid maps for both plasmids listed under the ‘basic rules for the assignment’
 The translated sequence of your coding region (using ORF Finder software) aligned with the coding sequence
 The translated sequence of your full length fusion protein (using ORF Finder software) aligned with the coding sequence
 A theoretical tryptic digest of your untagged protein (no missed cleavage sites)
 A theoretical tryptic digest of your tagged/fused protein (no missed cleavage sites)
 Results from MASCOT to demonstrate that the tryptic peptides obtained from your untagged protein actually correspond to your allocated cDNA sequence (you need to show results from 2 peptides)
Basic rules for the assignment
 You must use the following plasmids (details available online) for cloning:
i). pEGFP-N1
ii). pH6HTN His6HaloTagT7
 Your encoded protein must be expressed as a full length fusion protein after being cloned into each of the above plasmids.

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